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2. Bake slides for 1 h in a 60°C oven.
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When you're ready to bake, slide the tin on to the hot baking tray.
Sections were baked onto slides overnight at 55°C.
Sections (4 μm) were placed on Superfrost Plus slides, baked 1 h at 60°C and deparaffinized for 5 minutes in Citrisolv for X-Gal-stained tissues.
Sections (3 μm) of formalin-fixed, paraffin-embedded tissues were cut onto DAKO TechMate™ S2024 charged slides, baked in a 60°C oven overnight to maximise section adhesion, dewaxed in xylene, and rehydrated through a series of alcohol to water.
Three-micrometer-thick sagittal sections from the medial joint compartment of the right knee were cut on a microtome and mounted on positively charged slides, baked at 60°C for 30 minutes, deparaffinized in xylene, and rehydrated in decreasing concentrations of ethanol.
In brief, a combination of sequential baking and UV crosslinking was implemented where slides were baked for 80 min at 80°C in a drying oven without vacuum.
MPTMS-functionalized slides were baked at 80°C for 30 min to strengthen the glass-silane bond and kept in ethanol until use.
The slides were baked at 60°C for 12 hours and aged at room temperature for 72 hours.
Briefly, slides were baked at 55°C for 45 min, deparaffinized in three washes of xylene, and rehydrated in a decreasing ethanol gradient.
Slides were baked at 56°C for 2 days, then deparaffinized and incubated for 40 minutes at 96°C in 10 mM sodium citrate buffer (pH6).
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