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Similarly to UV photolysis experiments 1 control bag was kept in the dark by covering it with aluminum foil (dark control) and one was left uncovered.
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Following spawning, the bags were kept about 15 cm apart in a crop room; with the temperature maintained around 25 °C30 °C and humidity ranging from 70 to 90%%.
Once the sawdust has cooled, Sorghum grain spawn is added, and the bags are kept in moderately dark conditions.
Retrieved seed bags were kept in a plastic bag at room temperature in the dark for three weeks or less prior to the germination assay.
Bags were kept in an uncontrolled greenhouse under natural temperature and light.
Zip-lock bags were kept in a sealed plastic box containing desiccant (anhydrous CaSO4 granules).
The spawned bags were kept in dark at 23 25°C till complete mycelial covering.
The bags were kept in 0.1 M H2SO4 in a refrigerator for a day until brought to the field site and inserted into the heaps.
Bags were kept closed for at least 10 min and the contents of all thorns were then collected in 70% ethanol, transported to Germany, and counted using a binocular microscope separately for larvae (four stages separated according to size and structure), pupae, workers, and alates.
Ensure all bags are zipped closed when carrying them, souvenir bags are kept close and stored in other sealable bags, or the "Disneyland Shopping Service" storage and collection facilities are used where possible.
However when the owner of the shop was tracked down by the BBC, she insisted that Winfrey was "absolutely allowed" to examine the bag, which was kept behind a screen.
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