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For the combined micro- & mesofauna isolation under the Baermann funnel method, we used three different sieves in a vertical series.
The Baermann Funnel method is most often used to capture Nematoda, but will also capture most other types of motile micro- & mesofaunal populations, as it depends on organismal locomotion40,41.
Three segments were placed in a 9-cm-diameter Petri dish. A. besseyi individuals were collected from rice panicles by using a modified Baermann funnel method, and the surfaces of the nematodes were sterilized with streptomycin sulfate (0.l %) for 10 min.
Nematodes were extracted over 48 hrs using a modified tray method version of the Baermann funnel method within 5 days from collecting the samples.
Nematodes were isolated with the Baermann funnel method [49] and the nematodes could pass through two or three layers of filter-paper while fungal mats could not.
The fungal-feeding nematodes were cultivated as described above, and harvested by the Baermann funnel method (Staniland 1954).
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Hence, the aforementioned laborious techniques seem to be justified and a combination of the Baermann funnel and the Koga agar plate method may lead to the most accurate results.
This collected soil was then used for both Baermann funnel and sugar flotation extraction.
Viglierchio, D. R. & Schmitt, R. V. On the Methodology of Nematode Extraction from Field Samples - Baermann Funnel Modifications.
The accumulated material from each sieve was removed by spatula and placed together onto cotton gauze in a Baermann funnel for further extraction followed by sugar flotation40.
The microwave method recovered 97.8% of introduced adults, while 90.6% of adults were recovered by the Berlese funnel method.
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