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The composition of each bacterium in the sample plaque was calculated as the percentage ratio of its cfu count formed on the selective culture plate compared with the total cfu formed in the universal plates.
The most abundant bacterium in the sample, Faecalibacterium prausnitzii, is thought to be protective [ 38], but there is currently no accepted analysis strategy that would allow one to make certain conclusions about the medical relevance of the results.
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In 12.5% of the cases, PCR afforded identification of bacteria but conventional methods showed no bacteria in the sample.
Culture techniques are designed to promote the growth and identify particular bacteria, while restricting the growth of the other bacteria in the sample.
One possible reason is that other motile bacteria remained under detection level of HOMIM analysis that discloses predominant bacteria in the sample.
These studies typically use sequence variation in a short fragment of ribosomal genes (e.g. 16S rDNA) for estimating the diversity of bacteria in the sample.
The resolution of the system was high enough to allow easy identification of individual TB bacteria in the sample, as well as to observe the standard rod-shaped morphology.
The positive band indicated the presence of bacteria in the sample.
Materials from samples were also directly Gram stained to detect any bacteria in the sample before cultivation.
We also tested the cDNA samples purified with only bacteria in the sample for the same reason, and no amplification was found in either case.
A significant number of microorganisms do not grow in selective cultures, and fast-growing bacteria can mask other clinically important bacteria in the sample [ 43].
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