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The contribution of a gene to the fitness of a bacterium can be assayed by whether and to what degree the bacterium tolerates transposon insertions in that gene.
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The density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be assayed on a single microarray with high resolution.
Cells exposed to the bacteria are subsequently transformed with the sequence of interest and can be assayed for expression a few days after infiltration.
Therefore, sequence-dependent translocation of the hybrid protein into calmodulin-containing cells can be assayed by simply measuring cyclase activity after allowing contact of bacteria with host cells.
Proteins identified in our analysis, which are absent in M. avium and M. bovis and do not show any significant identity with proteins from lung infecting bacteria can be shortlisted for developing various immunological assays to identify M. tuberculosis, on the basis of their seroreactivity and abundance.
Assays using bioluminescent bacteria can be divided into two groups, namely systems with constitutive expression and systems with inducible expression.
These bacteria can be killed by irradiating meat, Dr. Duchin said.
Some bacteria can be worse.
(Even antibiotic resistant bacteria can be killed by sufficient heat).
These bacteria can be present in food.
Mesophile bacteria like lactic acid bacteria can be inactivated at 200 600 MPa about 5 60 minutes.
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