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For each samples bacteriological tests were carried out in order to verify the level of contamination on sample of PRP obtained at the end of the processing.
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Bacteriological and molecular tests were carried out by the microbiology laboratories of the respective collaborating hospitals.
Between 1946 and 1958, 67 tests were carried out.
Two tests were carried out.
All tests were carried out blindly.
A routine bacteriological examination was carried out (by AHVLA Winchester, Hampshire, UK) if the SCC exceeded 10 cells per ml.
Questionnaires, observation of disinfection and bacteriological tests were performed in each center (decontamination tanks, gastroscope, storage location).
Retrospective analysis of bacteriological tests has been carried out as well as a susceptibility evaluation of cultured bacterial and fungal strains to antibiotics over a five-year period in subjects treated at the Cranio-Maxillo-Facial Clinic in Katowice.
All bacteriological analyses were carried out according to the Standard Method for the Examination of Water and Wastewater (APHA et al. 1998).
The bacteriological samples were carried to the laboratory in freezing condition (4 °C).
Direct saline wet mount, Gram staining and bacteriological cultures were carried out for all specimens.
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