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Based on antibiotic treatment in patients when bacteriological specimens were obtained, two groups were defined: no antibiotic group and antibiotic treatment started before PSB group.
Bacteriological specimens were cultured on standard media.
P. aeruginosa is not generally considered to be part of the normal human flora [ 16], and in most patients admitted to hospital for the first time, P. aeruginosa is not usually isolated from bacteriological specimens until the patient has been in the hospital for several days [ 22, 24, 25].
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The available guidelines addressing management of MDR/XDR-TB in children specifically recommend treatment according to the susceptibility of the index case strain if it is not possible to obtain a bacteriological specimen from the child [ 228, 229].
One of the main difficulties is obtaining adequate specimens for bacteriological confirmation of the disease in children.
Necropsy and bacteriological analysis on tissue specimens from kidney, spleen and central nervous system (CNS) were carried out according to standard procedures.
Of the variety of methods for obtaining clinical specimens for bacteriological confirmation of paediatric tuberculosis, gastric aspiration to obtain swallowed bronchial secretions is the most commonly used [ 25- 27].
The freshly obtained bone is stored on a special "hold" shelf until a bacteriological report of the specimen is obtained from the laboratory, releasing it for use.
While the bacteriological spectra of PTA patient specimens were generally reported without comparison to other patient groups, data from RT patients were compared with data from healthy persons or patients undergoing tonsillectomy because of tonsillar hypertrophy [4], [14], [17] [19].
In addition, bacteriological analyses of respiratory tract specimens were conducted to determine patient infectious status.
Forty eight non-duplicate carbapenem resistant P. aeruginosa isolates were obtained from clinical specimens submitted for bacteriological testing from hospitalized in-patients admitted to Kasr Al Aini Hospital (KAA) and National Cancer Institute (NCI), Cairo University, Egypt from January 2011 to January 2012.
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