Exact(12)
Standard bacteriological procedures according to Bergey's Manual of Systematic Bacteriology [ 27] were employed.
Following international recommendations38, samples from target tissues underwent specific bacteriological procedures to screen Listeria spp. and Brucella spp. (Case 1 and 2).
Organisms were identified according to routine bacteriological procedures.
The enterococci were isolated and identified from stool sample using standard bacteriological procedures.
Brucella field isolates were further analysed using both molecular and standard bacteriological procedures.
TM conceived the study design, performed bacteriological procedures, participated in experimental work, data analysis and drafted the manuscript.
Similar(48)
The bacteriological procedure was done as outlined by Balows et al. [ 13].
Time taken at different stages of MDR-TB management using conventional culture and DST method, starting from sample collection to start of treatment, was also reported in another study which presents a total turnaround time of 5 months which was almost double of the bacteriological procedure [ 35].
After 24 hours of incubation at 37°C, bacteriological routine diagnostic procedures including selective enrichment and biochemical confirmation (API20E, bioMérieux, Nürtingen, Germany) took place as described previously for Enterobacteriaceae, especially Escherichia coli (E. coli), Klebsiella species (spp ., Enterobacter spp., Citrobacter spp., and Salmonella spp. [ 24].
However, current bacteriological monitoring procedures which focus on the quantification of faecal indicator organisms (FIOs) as a proxy for microbial pollution may not be representative of total bacterial contamination levels present in shellfish harvesting areas.
On the basis of clinical data, patients with clinical suspicion of VAP should be further evaluated by imaging procedures, bacteriological cultures, and biomarkers.
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