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In this study, primers designed for the Campylobacter jejuni cadF gene sequence were used in a SYBR Green I real-time PCR assay as an alternative to a conventional bacteriological method for the rapid detection of C. jejuni from poultry.
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While traditional labour-intensive bacteriological methods for the detection of M. mycoides subsp. mycoides have been replaced by molecular genetic techniques in the last two decades, these latter approaches require well-equipped laboratories and specialized personnel for the diagnosis.
The determination of total coliform and total faecal coliform count is the most widely used bacteriological methods for assessment of the quality of drinking water.
Our aim was to assess the diagnostic value of different non-invasive (C-UBT) and endoscopy-based diagnostic methods (visual endoscopy, classical cytological and histological examination of mucosal specimens, PCR and bacteriological methods) for monitoring patients after eradication therapy in a late post-treatment setting.
In total, 2.02% of the samples were Brucella positive, by both bacteriological method and the novel qPCR method.
The bacteriological method, which is generally used for assessing the multiplicity of mycobacterial infection in animal organs and tissues, involves inoculation of their homogenates on special agar media and counting colony-forming units.
Provincial hospitals do not have ELISA or bacteriological methods to confirm the diagnosis.
Bacteriological methods are suggested for detecting the putative antimicrobial resistance of H. pylori, aimed at successful eradication of infection in persistent peptic ulcer cases.
The standard bacteriological methods were used for detection of Pm in the nasal swabs and lung samples, as described previously [ 26].
The source of infection was represented by cattle, in which the infection was confirmed by bacteriological method.
Bacteriological analysis of sputa is the most widely accepted method for the diagnosis and assessing treatment response of pulmonary TB [ 1].
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