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In the event of a shutdown, he said, scientists must destroy all bacteriological experiments under way.
KNP carried out bacteriological experiments and hydrogen measurements and edited the paper.
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AA contributed to conception and design of the experiment, sampling and bacteriological analysis, data analysis and drafting of the manuscript.
TBJ contributed to conception and design of the experiment, sampling and bacteriological analysis and drafting of the manuscript.
The udders of the cows were pathogen-free prior to the experiment as determined by bacteriological examination of quarter milk samples 1 week before the start of experiment.
Bacteriological analyses and real-time quantitative (q PCR experiments were applied to all samples.
ALM designed and supervised the experiment as well as the bacteriological analyses and drafted the manuscript.
Bacteriological examination of milk samples taken before the experiment indicated these animals were not infected with any major mastitis-causing pathogen prior to the experiment.
In confirmation of these previous experiments, five of six piglets with initially healthy lung had bacteriological and histological evidence of lung infection after 60 hours of mechanical ventilation.
In all experiments, bacteria were grown in SM medium (10 g l−1 bacteriological peptone, 1 g l−1 yeast extract, 16.2 mM KH2PO4, 5.7 mM K2HPO4 and 4 mM MgSO4) supplemented with 1% glucose.
Imipenem recoveries in the three media were determined in each rat by retrodialysis by drug before imipenem administration (30 mg/kg over 30 min intravenously), then microdialysis samples were collected during 150 min. At the end of experiment, after euthanasia, the lungs were removed and a quantitative bacteriological study was performed to confirm pneumonia.
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CEO of Professional Science Editing for Scientists @ prosciediting.com