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The conventional method is the bacteriological assay, which is reliable but time consuming.
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External abscesses found during the experiment period were sampled through an incision with sterile surgical equipment, followed by drainage of purulent material into sterile flasks for bacteriological assays.
Regarding conduction of experiments, MD, SL-S, and MN performed the necropsy and evaluated the pathological records, and BPV and MM performed the immunological and bacteriological assays.
The discrepancy seen between the immunological and bacteriological assays is most likely due to not fully functional T cells from patients with TB.
We discuss the implications of our results for the study of bacteria in porous media and for the design of improved bacteriological chemotaxis assays.
The patient was clinically examined and we performed routine tests, immunologic assays, bacteriological swab and skin biopsy.
Fifty-one wildlife samples from 13 different species were also collected in the same area and tested with serological (lateral flow assay) and bacteriological (culture of lymph nodes) techniques.
Our objective was to evaluate the test characteristics of PathoProof ™ Mastitis PCR Assay and bacteriological culture (BC) in diagnosing bovine intramammary infections caused by S. aureus at dry off at different PCR cycle threshold (Ct -value Ct -value
The disadvantage might be that, for example, for bacteriological findings or hormonal assays – data might be only from preselected animals.
The laboratory assays for bacteriological confirmation and the markers of infection were performed by investigators blinded to the clinical data.
LDR carried out the herd screening, vaccination and sampling, performed the bacteriological isolation and serological assays, participated in the statistical analysis.
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