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In the case of death or euthanasia, pigs were necropsied, lungs and heart were collected and bacteriological analyses were performed.
Samples (100 g) were collected over a 42-day period at 3-day intervals, kept at 6°C and bacteriological analyses were performed within 4 h of collection.
Bacteriological analyses were performed at the Veterinary School of Toulouse (UMR 1225 laboratory) by conventional techniques according to International Dairy Federation (1981) guidelines, with a few additions that have already been described in Rupp et al. [ 22].
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Analyses were performed in R v.3.2.2.
All statistical analyses were performed in R50.
Statistical analyses were performed initially by ANOVA.
The analyses were performed in triplicate.
Immunocytochemistry analyses were performed as previously described11.
For each phenotype, two analyses were performed.
Analyses were performed in transmission mode.
Statistical analyses were performed using GraphPad Prism.
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