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In case of prolonged incubation time in the open wound, no bactericidal effect was reached (data not shown).
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In present study, a significant bactericidal effect was achieved in the opsonophagocytosis assay and the serum bactericidal assay (Fig. 5), indicating a phagocytosis and classical complement pathway killing mediated by the antiserum.
A 102 103 CFU bactericidal effect was also seen with HT61 but Mupirocin barely had any effect.
The bactericidal effect was totally reversed in presence of isoleucine; leucine, valine and pantothenate indicated the specificity of antisense induction.
At this time, a complete bactericidal effect was observed.
The only exception was at 100% concentration, where bactericidal effect was observed.
However, the highest bactericidal effect was detected against S. aureus (MIC = MBC = 3.12 mg/mL).
Against other strains, reliable bactericidal effect was maintained at 4x MIC at 24 h after incubation.
The classic mechanism of defensins' bactericidal effect is the "pore formation" theory.
A bactericidal effect is desired because, to put it succinctly, dead bugs don't mutate.
Bacteria strains possessing the bactericidal effect were chosen to perform time-kill assay.
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CEO of Professional Science Editing for Scientists @ prosciediting.com