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Vancomycin bactericidal effect was defined as ≥99.9% growth inhibition in colony count compared with the starting inoculum count [ 30].
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A bactericidal effect is defined as a 3 Log decrease in the CFU/ml or a 99.9%% kill over a specified time [ 25].
If the ratio MBC/MIC is 1 or 2, the effect was considered bactericidal but if the ratio MBC/MIC is 4 or 16, the effect was defined as bacteriostatic [ 30].
If the ratio MBC/MIC = 1 or 2, the effect was considered as bactericidal but if the ratio MBC/MIC = 4 or 16, the effect was defined as bacteriostatic [ 24].
A bactericidal effect is desired because, to put it succinctly, dead bugs don't mutate.
In present study, a significant bactericidal effect was achieved in the opsonophagocytosis assay and the serum bactericidal assay (Fig. 5), indicating a phagocytosis and classical complement pathway killing mediated by the antiserum.
A 102 103 CFU bactericidal effect was also seen with HT61 but Mupirocin barely had any effect.
The bactericidal effect was totally reversed in presence of isoleucine; leucine, valine and pantothenate indicated the specificity of antisense induction.
At this time, a complete bactericidal effect was observed.
The only exception was at 100% concentration, where bactericidal effect was observed.
However, the highest bactericidal effect was detected against S. aureus (MIC = MBC = 3.12 mg/mL).
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