Sentence examples for bacterial transformants which were from inspiring English sources
Briefly, cDNA for seeding the amplifications were obtained by directly transferring into the reactions 5 μl of cDNA bacterial transformants, which were grown in 1.2 ml of 2X YT and 0.005% chloramphenicol in 96-deepwell plates for 24 hours at 37°C.
To minimize positional effect of reporter gene expression, at least 1000 transformants on the membrane were collected to create a mixed population of transformants, which were cultured and assayed for the strength of GFP florescence.
Moreover, it enabled the recombinant enzyme to participate actively in bacterial metabolism, which was reflected by the growth of transformants in a glucose-containing medium.
Most of the transformants displayed a higher chitinase activity than the wild strain, and activity was highest for transformant Rc4-4, wasch was 2.4-fold higher than that of the wild type (Table 3).
Transformants were then plated onto LB/ampicillin/X-Gal plates, which were incubated at 37°C overnight.
Fast growing leucine prototrophic transformants were selected, which are expected to carry multiple copies of the expression cassette [ 24].
Nevertheless, different modes of transgene silencing might affect the screening efficiency of transformants in plant species, which is assumed to cause difficulty in obtaining transformants.
Unlike pSY6catP, pHT3 transformed at a high efficiency of 1.8 × 10 transformants μg-1 DNA, which is comparable to pMTL85141ermB.
The transformants had stable genetic phenotype which was genetically fingerprinted by RAPD analysis.
Bacterial transformants were selected on solid LB plates (15 g L−1 agar), supplemented with ampicillin (50 mg L−1), for 16 h at 37°C.
