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Bacterial transformants were selected on solid LB plates (15 g L−1 agar), supplemented with ampicillin (50 mg L−1), for 16 h at 37°C.

Bacterial transformants were selected on LB agar plates (15 g L−1 agar) supplemented with 50 mg L−1 ampicillin for 16 h at 37 °C.

Bacterial transformants were selected for on LB 2% agar plates supplemented with either 100 μg/ml ampicillin sodium salt or 60 μg/ml kanamycin sulfate.

Transformants were selected on selective minimal medium as described above.

The resulting verified plasmid was transformed into G27 and transformants were selected on Kan 25.

The transformants were selected on a minimal selective medium.

The transformants were selected by 5-FOA counter selection.

The transformants were spread on SPASC medium for selection, and several transformants were selected, based on colony size.

DY8430 was transformed with pAW8 and Leu+ transformants were selected.

The plant transformation was performed as described previously59, and the transformants were selected for kanamycin resistance on MS medium.

The construct was transformed into tpx mutant by electroporation and gentamicin resistant transformants were selected.