In vitro properties such as bioactivity and anti-bacterial tests of both coatings were investigated by soaking test in simulated body fluid (SBF) and bacterial formation, respectively.
In vitro anti-bacterial test of both coatings were carried out by microbial adhesion for Gram positive and Gram negative bacteria.
Bacterial testing has a number of benefits including low cost, quick test time, straightforward test procedures and good correlation with lengthy rodent carcinogenicity studies [23].
In vitro bacterial susceptibility testing of P. aeruginosa isolates was performed by use of disk diffusion tests following the EUCAST guidelines [ 22- 24].
The bacterial test panel consisted of 27 preserved strains or clinical isolates previously determined by cultural and molecular identification by the Diarrhea Department, National Institute for Infectious Disease Control and Prevention (DD-ICDC).
Against all bacterial test strains, the combination of NCT and H2O2 showed a more rapid killing than the single compounds.
Flasks were allowed to equilibrate to ambient room temperature for at least 10 min. One ml of bacterial test suspension was added to each flask.
For the Qiaxcel-B assay, each reference strain of bacterial test panel (Table 2), except for Vibrio cholera, was serially diluted 10-fold with 0.9% NaCl in the logarithmic phase of growth.
In addition, the bacterial distribution tests of ST4, ST7 or ST8 strain in animals within the implanted tumors were investigated.
