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The large-scale engineering of novel bacterial systems is a complex, challenging task.
Will the bacterial systems be as popular as the yeast n-hybrid systems?
They also perform some key posttranslational modifications not possible in bacterial systems.
This is because of the efficient heterologous production, high specific activity, and less stringent pH requirement of bacterial systems.
Also, expression differences between yeast and bacterial systems could be a factor explaining the dissimilarities in specific activity measurements.
These measurements reveal that functional complexes exhibit dynamic behaviors and thermodynamic stabilities distinct from those observed for bacterial systems.
Whereas high production was achieved with bacterial systems, metabolic engineering of yeast for butanol production is in the beginning.
Many methods designed to identify these interactions have been reported, including yeast two hybrid and plasmid-based bacterial systems.
Our bacterial sumoylation system includes several improvements over the existing bacterial systems.
We have developed a novel one-step purification system that accelerates untagged recombinant protein purification from bacterial systems.
In bacterial systems, the P-protein is composed of two subunits that are encoded by two distinct genes.
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