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The bacterial suspensions were obtained by making a saline suspension of isolated colonies selected from 18 to 24 h of nutrient agar plating.
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Homogenization of the bacterial suspension was obtained by vortexing or in an ultrasound water bath to disperse clumps.
Bacterial nanocellulose/CMC (carboxymethylcellulose) fibres suspensions were obtained from JeNaCell GmbH (Jena, Germany) in three different average fibre lengths (cf. Table 1).
Cell suspensions were obtained as described before.
Single cell suspensions were obtained from MLN and spleen.
Single cell suspensions were obtained using trypsin-EDTA.
Following treatment, single cell suspensions were obtained.
The nuclei suspension was obtained by centrifuging the suspension at 1100 rpm for 6 min.
Bacterial suspensions were heated at 65 , 75and 80 °C and samples for viable counts were obtained after 1,2,3 and 10 minutes at each temperature.
Bacterial suspensions were diluted in sterile Muller Hington Broth (MHB) to obtain a final inoculum of 106 CFU/ ml.
Bacterial suspensions were infiltrated into plants leaves using vacuum.
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