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Because no bacterial steps are required, large amounts of dbDNA can be made quickly, which makes it easier and more economical to create therapeutic products.
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Moreover, additional bias may be introduced e.g. by the bacterial cloning step.
We used the recovered DNA to transform high-efficiency competent bacteria and recovered the plasmids as bacterial colonies (Step 6).
Third, the bacterial cloning step may introduce a systematic bias in the observed haplotype frequencies due to differences in the compatibility of clones with the host's lifecycle.
In addition to its great improvement of efficiency and speed, NGS platforms can eliminate the bacterial cloning step that can bias the composition of the cDNA library.
These new technologies eliminate the bacterial cloning step used in traditional Sanger sequencing; instead, they amplify single isolated DNA molecules and analyze them with massively parallel processing.
In addition to advantages with regard to cost and speed, another major advantage of the NGS platform is elimination of the bacterial cloning step that can bias the composition of the cDNA library.
Recently developed microbead technologies capable of sequencing hundreds of thousands of DNA molecules in parallel provide a way to obtain genomic information from these species for reasonable cost, and without any bacterial cloning step.
When BAL fluid analysis rejected the diagnosis of bacterial pneumonia, additional steps followed to determine an alternative diagnosis.
Infectious diseases caused by bacteria are characterised by several discrete steps: bacterial adhesion to the host, colonisation within or on the host, and evasion of host defences [ 31, 49].
L-arabinose utilization in S. cerevisiae has been achieved by introducing multi-step oxidoreductive fungal or multi-step bacterial pathways.
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