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Bacterial solutions were then washed and prepared in the same manner described for BDR media above.
The solution was washed with HCl 0.1 n (3×10 mL).
The solvent was washed with 1% NaCl (1 1) solution.
The harvested bacterial cells were washed three times with saline solution (8.5 g/l KCl), and re-suspended in 50 mM potassium phosphate buffer (pH 7.5).
The bacterial pellets were washed twice and diluted in an isotonic saline solution to obtain an optical density of 0.63 to 0.65 nm determined by spectrophotometry [ 17].
Bacterial lawns were washed off of NGM plates with water.
The formed bacterial nanocellulose gels were washed with 2% aqueous NaOH solution at 70°C for 10 min, and then washed repeatedly until a neutral pH was obtained.
The bacterial pellets obtained were washed twice with PBS.
The surface exposed to the bacterial solution was placed in the 37 °C incubator for 30 min. Then the surfaces were washed with PBS (three times) to remove non-specific-bound bacteria.
After digestion solution was removed, tissue pieces were washed once with DMEM and digested with crude bacterial collagenase (type IA, C 2674; 1 mg/ml; Sigma, Saint Louis, MO USA).
PI solution was removed and cells were washed twice.
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