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Finally, 0.7 ml of this bacterial solution was mixed with 0.5 ml glycerol and the bacterial strains were frozen at −80°C.
Cells were aspirated, then incubated at room temperature for 15 min. One hundred microliters of bacterial solution was mixed with 25 μL of ONPG (10 mg/mL), and the cells were incubated at room temperature until the mixture produced a yellow color.
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A stock solution was mixed with 500 µl of OP50 overnight bacterial suspension in a 1 10 ratio.
The solution was mixed thoroughly and incubated at room temp for 15 min. The bacterial suspension was pelleted (16,000×g for 5 min) and 200 µl of the supernatant was added to new flat-bottom 96-well plate.
Then solution was mixed.
The solution was mixed overnight at 25 °C.
Then this solution was mixed with above solution.
Fuel solution was mixed with oxidizer solution which was under stirring followed by mixing of surfactant solution.
The solution was mixed by pipetting.
The solution was mixed thoroughly at 0 °C.
were added, and this solution was mixed again.
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