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The bacterial sequences in this cluster come from groups that are not typically considered as having GRXs (see caption for Figure 1A).
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To this end, we selected the bacterial sequences in the complete STAP database which had more than six levels of taxonomic annotation.
We identified 14 most abundant phyla (represented by more than 1 % total bacterial sequences) in the freshwater sponges and its surrounding water (Fig. 1).
There is however, a significant bias towards bacterial sequences in the RAST and MG-RAST database for comparison, hence all hits identified below were explored to make sure that if the RAST homologue was bacterial, that there was the potential for a eukaryotic homologue in the NCBI nr database.
The effective bacterial sequences in the four samples were all assigned to corresponding taxonomies by using BLAST combined with MEGAN.
In the latter approach, 53 conserved genes encoding bacterial ribosome protein subunits (rps genes) are identified and compared with all bacterial sequences in the database.
To identify potential viral or bacterial sequences in the NLB sample, 210,752 tags were sequenced and were directly matched with the microbial virtual tag library.
By contrast, the majority of bacterial sequences in the wallaby gut belong to the Firmicutes phylum, similarly to what is observed in human and mouse guts.
There were only 10 hits at an E value of less than 10−20 toward bacterial sequences in the cDNA library as determined using Blastn (Supplementary Table S2).
The strongly supported co-grouping of fungal and bacterial sequences in the ChNPS11/ETP/ChNPS12 group, as in the outgroup adenylating enzymes (Fig. 2, Additional file 6A-C) suggests this is a tenable hypothesis.
We also aligned the M1-P-M2 region of KcsA with the ten bacterial sequences in the microbial sequence database that were most similar to KcsA to produce a KcsA-like family.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com