Sentence examples for bacterial purification from inspiring English sources

RNA from bacteria and L929 cells were extracted simultaneously to avoid changes in transcriptomic profile after the bacterial purification process.

In order to determine eukaryotic cellular contamination following bacterial purification, non-infected cells were processed in parallel as a control.

Bacterial purification, DNA extraction and pulsed-filed gel electrophoresis were performed as described in Additional file 15 [see Additional file 15].

For bacterial purification, proteins were expressed with an N-terminal 6× HIS tag in Rosetta2 DE3 pLysS competent cells (QB3 MacroLab, UC Berkeley) by induction with 100 µM IPTG for 18 hr at 16°C.

Genomic and plasmid DNA isolations were performed using the Tiangen Bacterial Genomic Purification Kit (Tiangen, Beijing, China) and the Plasmid Minipreps DNA Purification System (Tiangen, Beijing, China), respectively.

This paper presents the bacterial expression, purification, and kinetic characterization of the soluble CA domain of AaCA1.

We also report the cloning, bacterial expression, purification and characterization of Henipavirus NTAIL and PNT domains.

We then report the cloning, the bacterial expression, purification and characterization of Henipavirus PNT and NTAIL domains.

The MICROBExpress Bacterial mRNA Purification Kit (Ambion) was used to deplete the pool of 16S and 23S rRNA molecules present in the sample.

We thank J. Miceli for expert technical assistance throughout, J.Hassen for the construction of the S6A mutant, and Dr. D. Worthylake for technical advice on large-scale bacterial protein purification.

As the recombinant ASC PYD is expressed insolubly in bacterial cells, purification requires denaturation of inclusion bodies and protein refolding.