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The bacterial pellets were washed twice in phosphate buffer saline (PBS) at 5000 rpm for 5 min.
Bacterial cells were harvested by centrifugation at 1,000 × g for 5 min and the bacterial pellets were washed with sterile water.
Bacterial pellets were washed in PBS and TES buffers, inactivated at 80°C and stored lyophilized until DNA isolation.
Actively growing or nonculturable Y. pestis microcosms were harvested by centrifugation and the bacterial pellets were washed three times with PBS.
After centrifugation at 3,000 g for 10 min, the bacterial pellets were washed two times in isotonic saline solution and resuspended in isotonic saline solution to yield an appropriate number of CFU per ml (determined by OD measurement at 600 nm).
The ON culture was centrifuged at 14000 rpm for 15 minutes and the bacterial pellets were washed twice in PBS.
Similar(52)
The bacterial cells were centrifuged at 12,000g for 5 min and the bacterial pellet was washed twice and resuspended in sterile NaCl (0.3 %).
The bacterial pellet was washed twice with 30 ml of sterile water and the cells were resuspended in glycerol 30%.
One millilitre of bacterial culture was centrifuged at 8000 x g and the bacterial pellet was washed thrice with 1x PBS and then resuspended in 500 µl 1x PBS.
Then, the bacterial pellet was washed three times with diluted water to extract the bacterial toxin before optical density (OD) assessment.
The bacterial pellet was washed, resuspended in protein extraction buffer [50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 10% glycerol (v/v), 1 mM DTT, 1 mM PMSF] and lysed using a French Press.
More suggestions(17)
bacterial pellets were centrifuged
bacterial pellets were used
bacterial pellets were examined
bacterial pellets were stored
bacterial pellets were homogenized
bacterial pellets were separated
bacterial pellets were solubilized
bacterial pellets were diluted
bacterial lawns were washed
bacterial pellets were re-suspended
bacterial pellets were suspended
bacterial pellets were sonicated
bacterial suspensions were washed
bacterial pellets were collected
bacterial pellets were harvested
bacterial pellets were lysed
bacterial cells were washed
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