Sentence examples for bacterial pellets were sonicated from inspiring English sources

Harvested bacterial pellets were sonicated in phosphate-buffered saline (PBS) containing 4 mM DTT and a cocktail of protease inhibitors (Roche).

Bacterial cell pellets were sonicated and washed with deoxycholate following resuspension in 10 mM Tris pH 8.0 with 1 mM MgCl2, 200 µg DNAse, and Complete Protease Inhibitor Cocktail (EDTA-free) (Roche, Indianapolis, IN).

To detect chromosomal flanking regions, pellets were sonicated (Bioruptor sonicator) to obtain DNA fragments of 100 400 nt.

Pellets were sonicated to complete lysis.

Cell pellets were sonicated in buffer D and, following centrifugation, MgCl2 (15 mM final concentration) was added to the supernatant.

After fixation and lysis, DNA pellets were sonicated into chromatin fragments of an average length of <500 bp.

Both crude extracts and pellets were sonicated again, this time in the presence of 1% SDS.

The harvested insect cell pellet was sonicated in lysis buffer.

The cell pellet was sonicated at 300 W (amplitude 100%, pulse 5 s/10 s, 2 min) to obtain the cell lysate.

The pellet was sonicated for 30 seconds and incubated on ice for 30 minutes.

The pellet was sonicated in 0.2 volumes of 8 M urea buffer (fraction 5).