Sentence examples for bacterial pellets were solubilized from inspiring English sources

After incubation, bacterial cultures were centrifuged and bacterial pellets were solubilized using Laemmli buffer.

The bacterial pellets were solubilized with lysis buffer (7 M urea, 2 M thiourea, 4% CHAPS, and 30 mM Tris-HCl (pH 8.5)), and centrifuged at 360,000×g for 1 h at 10°C to eliminate genomic DNA.

FP bacterial pellets were solubilized (50 mmol/L potassium phosphate, 400 mmol/L NaCl, 100 mmol/L KCl, 10% glycerol, 0.5% TritonX-100, 10 mmol/L imidazole, 1 500 Calbiochem protease inhibitor cocktail III), sonicated, and centrifuged (15,000 g, 4°C, 10 min).

The rHsp65 present in the bacterial pellet was solubilized with 6 M urea and submitted to preparative SDS-PAGE gel.

Pellets were solubilized in sample buffer [97] and analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) using 10% acrylamide in the resolving gel.

After TCA-precipitation pellets were solubilized in buffer A containing 1% SDS (20 min at 37°C), and diluted 10 times with buffer A (containing 0.2% Triton X100).

The resultant pellets were solubilized, and protein concentrations were measured.

After washing, the pellets were solubilized in lysis buffer and subjected to electrophoresis as described above.

The pellets were solubilized in 9M urea, 2% Triton X-100, and 1% DTT.

The supernatant was discarded and the resulting protein pellets were solubilized in SDS-loading buffer at 80°C for 20 minutes.

Mitochondrial pellets were solubilized in 200 μl 2% SDS, 100 mM DTT, and 100 mM Tris HCl, pH 7.4.