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Proteins from the supernatant and the bacterial pellets were separated and detected using rabbit polyclonal antibodies against YopE, YopJ and YopM, as described above.
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After a day of incubation, the culture was centrifuged at 10,000×g for 10 min, and the resulting bacterial pellet was separated and retained.
Pellets were separated using a sieve according to their size and counted.
The supernatants and pellets were separated by low-speed centrifugation.
Plasma and blood pellet were separated, weighted, and counted for radioactivity.
The culture supernatant and cell pellet were separated by centrifugation (4 °C, 10,000g, 10 min).
Plasma and the hematocrit pellet were separated and assessed for radioactivity.
After incubation, cells were harvested and supernatant and cell pellet were separated.
Supernatant and pellet were separated by SDS-PAGE.
Bacterial pellets were lysed in 30 mL lysis buffer, and homogenized by sonication.
The volumes of the supernatants and the bacterial pellets were normalized according to the OD600 of the bacterial cultures to ensure equal loading of the samples.
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