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After IPTG induction, the bacterial pellets were collected by centrifugation.
Bacterial pellets were collected from each flask by centrifugation and resuspended in 5 ml GST column binding buffer (10 mM Na2HPO4, 1.8 mM KH2PO4, 2.7 mM KCl, 140 mM NaCl2, pH7.6 and 4 mM PefaBloc from Roche Diagnostics GmbH, Mannheim, Germany) and frozen at -80°C.
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The bacterial cell pellets were collected at 8000 g for 10 min (4°C).
To determine bacterial numbers in the stools, faecal pellets were collected from individual mice, weighed, and homogenised in 1 mL of SPS.
Further pellets were collected and supernatants were discarded.
Cell pellets were collected at 1000 rpm at 4°C.
The cell pellets were collected for analyzing COX-2 expression.
The samples were centrifuged again, and the pellets were collected.
The exosome pellets were collected for characterizations and RNA extractions.
Subsequently, fresh faecal pellets were collected every 24 h.
Fecal pellets were collected from each rat at necroscopy.
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