The Bruker BioTyper, a simple matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer for identification of bacterial pathogens based on intact protein masses, "has been a smash success ….
The proposed methodology provides a universal platform for the detection of bacterial pathogens based on engineered peptides, as alternative to the most commonly used immunological and gene based assays.
The purpose of the present study is to investigate differences in the likelihood of emergence of fluoroquinolone-resistant mutants among major bacterial pathogens based on MPC determination and to characterize the resistance mechanism of mutants.
However, IRG-dependent resistance in mice has been claimed for many other protozoal and bacterial pathogens based always and only on mice deficient in Irgm1, the GMS subfamily regulator protein that does not localize to the T. gondii vacuole (Martens et al., 2005; Taylor, 2007).
Antibiotic therapy was studied and was considered effective if it exhibited appropriate in vitro activity and was appropriate for the pathogens isolated (or, in the case of culture-negative bacterial infection, for the suspected pathogens, based on international antibiotic therapy guidelines).
Bacterial growth was presumed to be these pathogens based on the presence of pigments (A. salmonicida), morphological characteristics and Gram stain results of re-isolated bacterial colonies.
Within our junior research group we want to develop an on-chip device to probe antibiotic susceptibility patterns of sepsis pathogens based on optical spectroscopy without the need for time-consuming bacterial cultivation.
Given the strong patterns in size and shape of pathogens, development of capabilities to recognize pathogens based on physical features, in addition to surface biomolecules, perhaps represents an evolutionary step that the macrophages utilize in fighting bacterial infections.
The aim of this study was to evaluate the optimal strategy for identification of bacterial pathogens in neonatal bacterial meningitis based on the three methods including RT-PCR, mPCR/RLB and CSF bacterial culture.
Evaluation of vaccine efficacy for protection against colonisation (VEcol) with Streptococcus pneumoniae and other bacterial pathogens is often based on a cross-sectional study design, in which only one nasopharyngeal sample is obtained per study subject.
