The bacterial loads in different tissues after treatment with NZ2114 at different time points were determined.
The bacterial loads in lung, spleen, and liver showed obvious dose-dependent effect (Fig. 4b d).
Additionally, the blood and lung were collected after 24 h post-treatment; bacterial loads in these tissues were calculated.
Meanwhile, the bacterial loads in blood showed sharp decrease and hardly detected at 16 h posttreatment with 10, 20, and 40 mg/kg NZ2114.
The bacterial loads in different organs were monitored at 2, 4, 8, 16, and 24 h postinfection after treatment with NZ2114 and control ampicillin.
The mice were sacrificed by cervical dislocation; bacterial loads in blood, lung, spleen, and liver were detected in 0, 2, 4, 8, and 16 h post-treatment.
Disruption of intravascular NETs did not, however, result in changes in bacterial loads in BALF, lung, blood, or liver (Figure 5).
After injection of bacteria, survival rate was monitored for 7 days; b Effects of NZ2114 on the bacterial loads in blood after 24 h post-treatment.
The bacterial loads in blood (a), lung (b), spleen (c), and liver (d) were detected in 0, 2, 4, 8, and 16 h post-treatment.
Moreover, bacterial loads in spleens and livers of such mice were also similar (Fig. 4C).
However, all the three genotypes produced similar bacterial loads in skin (P<0.05).
