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The bacterial isolates were subjected to different screening methods to obtain biosurfactant-producing strains.
The selected bacterial isolates were subjected to several biochemical tests as described by Holt et al. (1994) and Madigan et al. (2012).
All the bacterial isolates were subjected to FAFLP.
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Suspected Brucella spp. isolates were subjected to genomic DNA extraction by heat treating a loopful of bacterial material dissolved in MQ water at 99 °C for 15 min [ 22].
tuberculosis isolates were subjected to DST.
K. pneumoniae isolates were subjected to PFGE.
All the bacterial isolates were Gram stained and then subjected to standard analyses for classification as Gram positive (Catalase and Coagulase tests) and Gram negative (Oxidase test).
In the present study, only bacterial isolates were characterised (no human subjects, human material or human data were used).
From those membranes subjected to online salt cleaning, eight bacterial isolates were obtained from end element: three isolates represented 67%% of the total isolate count, two isolates were obtained from the lead element with one predominant isolates constituting 83%% of the count.
Six bacterial isolates were characterized by MALDI-TOF mass spectrometry.
These bacterial isolates were purified according to APHA, 9225B (1999).
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