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The identified bacterial isolates were plated onto casein milk agar plates and were incubated at 37°C for 24 h.
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Bacterial isolates were inoculated onto plates and incubated at 37 °C for 48 h.
Bacterial isolates were recovered by dilution plating on R2A agar and presumptively identified using 16S rRNA gene sequencing [ 29].
Firstly, the bacterial isolates were streaked on nutrient agar plates to obtain single colonies.
Colony morphologies of the bacterial isolates were evaluated on nutrient agar plates by using a stereomicroscope (Demirci et al. 2013).
Bacterial isolates were cultured on Mueller-Hinton agar plates with added sheep blood (Oxoid GmbH) and inoculated for 24 28 h at 37°C before diameters of inhibition zones were evaluated and interpreted according to the Clinical and Laboratory Standards Institute guidelines [ 22].
The selected bacterial isolates were tested qualitatively for lindane-degrading activity using spray plate method.
Bacterial isolates were routinely grown on 1% bacterial agar Brain Heart Infusion (BHI, Oxoid, UK) plates under anaerobic conditions in a Don Whitely Scientific Chamber at 37 °C.
Other bacterial isolates were typed and considered as significant if growth was in pure culture or dominating on the agar plate.
Six bacterial isolates were characterized by MALDI-TOF mass spectrometry.
Bacterial isolates were genotyped by pulsed-field gel electrophoresis (PFGE).
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