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The presence and distribution of 11 antibiotic genetic determinants from individual wastewater bacterial isolates were determined by the conventional polymerase chain reaction (PCR).
The radiation decimal doses (D10) values for all the bacterial isolates were determined (Table 3).
Phylogenetic positions of bacterial isolates were determined by sequence analysis of the complete 16S rRNA genes.
Antibiotic susceptibilities of all bacterial isolates were determined by the disc diffusion method (Oxoid, United Kingdom) using standard guidelines [ 21].
Antibiotic sensitivity patterns of bacterial isolates were determined by agar diffusion method using antibiotic discs [ 9] A total of 1,201 children with suspected septicaemia were studied.
12 The minimum inhibitory concentrations (MICs) of bacterial isolates were determined by E-test (AB Biodisk, Solna, Sweden) using the disc diffusion method following the Clinical and Laboratory Standards Institute (CLSI) guidelines.
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The capsule layer of the bacterial isolates was determined by negative staining.
Antibiotic susceptibility of pathogenic bacterial isolates was determined.
The testing of these bacterial isolates was determined not to be human subjects research by the Institutional Review Board of NAMRU-6.
The clonal relationship of bacterial isolates was determined by multilocus sequence typing (MLST) and the virulence of isolates was measured using cellular and animal models.
Optimum growth conditions of bacterial isolate were determined with respect to temperature and pH.
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bacterial isolates were analysed
bacterial isolates was determined
bacterial lysates were determined
bacterial isolates were received
bacterial isolates were selected
bacterial isolates were grouped
bacterial titers were determined
bacterial isolates were denominated
bacterial isolates were amplified
bacterial isolates were genotyped
bacterial burdens were determined
bacterial densities were determined
bacterial isolates were deposited
bacterial isolates were affiliated
bacterial loads were determined
bacterial suspensions were determined
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