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Eighteen (18) bacterial isolates were detected as positive hits and 5 (five) were considered as potentially interesting at pH 7.0, while 6 (six) at pH 9.0.
In our studies, bacterial isolates were detected in 31% of non-healing gaps compared with 16% in the uneventfully healing fractures.
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The NDM-1 gene of bacterial isolates was detected by PCR using specific primers (Kp-ndm1-F: 5′-ttcgacccagccattggcggcga-3′ and Kp-ndm1-R: 5′-atgcacccggtcgcgaagctgag-3′), as described previously [ 3].
No MRSA isolates were detected.
Six bacterial isolates were characterized by MALDI-TOF mass spectrometry.
These bacterial isolates were purified according to APHA, 9225B (1999).
The bacterial isolates were identified based on various tests.
The bacterial isolates were maintained in 40% glycerol at −80 °C.
Bacterial isolates were genotyped by pulsed-field gel electrophoresis (PFGE).
Total of 40 bacterial isolates were isolated from soil rhizosphere, of them three isolates showed the decolorization activity.
The fungus isolates were grown on potato dextrose agar medium, and bacterial isolates were grown on nutrient agar medium.
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