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The bacterial isolates were also identified using the VITEK 2 microbial identification system.
The bacterial isolates were also physiologically characterized on the basis of their growth at different temperatures, NaCl concentrations, and pH.
The bacterial isolates were also characterized on the basis of 16S rRNA gene sequencing to verify the recorded conventional characterizations of the isolates.
Two gene cassettes that encode proteins of unknown function, that have also been identified by other workers in class 1 integrons from a variety of clinical bacterial isolates, were also identified, orfF and orfC.
These bacterial isolates were also resistant to ampicillin, teicoplanin, and aminoglycosides but susceptible to linezolid, quinupristin/dalfopristin, and tetracycline.
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The metabolic pathway of BPA degradation by the bacterial isolates was also designed in today's analysis.
Radiation death of the bacterial isolates was also obtained by the linear regression equations.
Each bacterial isolate was also tested against a panel of six antibiotics using Kirby-Bauer disk diffusion method following CLSI guidelines [ 28].
Since some of the previous studies have shown that the lack of vaginal hydrogen peroxide (H2O2) producing lactobacilli is associated with bacterial vaginosis, the Lactobacillus isolates were also characterised for their production of H2O2.
Water bacterial community DNA samples and isolates were also directly screened for a range of resistance genes, class 1 integrons and incompatability group (Inc) A/C and IncN plasmids, using published primers and PCR protocols (Table S3).
K. pneumoniae isolates were also the first bacterial species shown to harbor the NDM-1 plasmid, a plasmid that harbors the New Delhi metallo-beta-lactamase (NDM-1) responsible for extensive carbapenem resistance (6).
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