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Molecular analyzes based on the identification of 16S rDNA, revealed that all the selected bacterial isolates were affiliated to Bacillus genus.
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A total of 55 non-repetitive isolates were affiliated to twenty species.
The 16S rRNA gene sequencing showed that hydrocarbon degrading isolates were affiliated to Rhodococcus sp., Achromobacter sp., Oerskovia paurometabola, Pantoea sp., Sejongia sp., Microbacterium maritypicum and Arthrobacter equi.
The remaining isolates were affiliated to the Firmicutes (7%), Actinobacteria (26%), and Bacteroidetes (2%) classes.
The remaining Gram-positive isolates were affiliated to the Firmicutes (31%), Actinobacteria (2%), and Bacteroidetes (1%) classes).
Using oligonucleotide arrays, S. aureus resistance and virulence genes were determined, and each isolate was affiliated to a clonal complex (CC).
Six bacterial isolates were characterized by MALDI-TOF mass spectrometry.
The bacterial isolates were identified based on various tests.
These bacterial isolates were purified according to APHA, 9225B (1999).
The bacterial isolates were maintained in 40% glycerol at −80 °C.
Total of 40 bacterial isolates were isolated from soil rhizosphere, of them three isolates showed the decolorization activity.
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