The bacterial isolates were identified based on various tests.
Bacterial isolates were identified using Analytical Profile Index (API, Bio Meriux, Craponne, France).
Bacterial isolates were identified using Analytical Profile Index (API, bioMeriux, Marcy-l'Etoile, France).
Bacterial isolates were identified using Analytical Profile Index (API, Bio Meriux, France).
The bacterial isolates were identified using API system (Biomerieux, Marcy l'Etoile, France) or Vitek 2 Compact (Biomerieux, Marcy l'Etoile, France) automated identification systems.
Based on morphological, biochemical, physiological, and molecular studies, the bacterial isolates were identified as Pseudomonas sp. (Cp1, 3, 5, and 13), Corynebacterium sp. (Cp2), Bacillus sp. (Cp4, 7, 9, 10, 12, and 15), Pectobacterium carotovorum (Cp6), Paenibacillus sp. (Cp8), Bacillus megaterium (Cp11), Bacillus pumilus (Cp14), and Terribacillus saccharophilus (Cp16).
Based on molecular characterization, the bacterial isolates were identified as Pseudomonas sp. (Cp1, 3, 5, and 13), Corynebacterium sp. (Cp2), Bacillus sp. (Cp4, 7, 9, 10, 12, and 15), Pectobacterium carotovorum (Cp6), Paenibacillus sp. (Cp8), Bacillus megaterium (Cp11), Bacillus pumilus (Cp14), and Terribacillus saccharophilus (Cp16) (Table 4).
Among these, 175 Gram-negative bacterial isolates were identified and recovered using standard microbiological procedures.
Bacterial isolates were identified by PCR for a panel of selected genes: 16S RNA, IS1081, Rv3120, Rv1510 and IS1245 [14], [16].
Bacterial isolates were identified using colony and Gram's staining morphology, followed by biochemical properties which were determined using the API biochemical test strip method API-BioMerieux, Marcyy l'Etoile, France).
Bacterial isolates were identified as H. pylori on the basis of Gram's stain, showing Gram-negative spiral forms, positive urease, oxidase and catalase tests as well as PCR amplification of H. pylori 16S rDNA [17].
