We used only purified clonal bacterial isolates, and did not need to perform isolations from patient derived samples.
Phylogenetic analysis of the bacterial isolates and their closely related species was performed for molecular characterization of the bacterial isolates.
In vitro susceptibility to antibiotics of the bacterial isolates and transconjugants was done by the Kirby Bauer disk diffusion method [17] on Muller Hinton (MH) agar.
Plasmid DNA from the bacterial isolates and marker plasmids of E. coli V517 were extracted using Wizard Plus SV Minipreps plasmid DNA Purification kit (Promega, USA) and was separated in 0.8% agarose gel at 70 volts for 4 h.
However, the unraveling of the correlation between the in vitro detection of Plant Growth-Promoting (PGP) traits in bacterial isolates and a real contribution to the host plant health in vivo is still lacking.
Citric acid, which was ammoniated to a pH of 4.0, was found to kill 100%% of all bacterial isolates, and is therefore an effective cleaning agent against biofilm bacteria.
Accordingly, descriptive statistics such as percentages and frequency distribution was used to describe/present bacterial isolates and antimicrobial susceptibility which was expressed as percent of resistant, intermediate and susceptible.
Accordingly, descriptive statistics such as percentages and frequency distribution were used to describe/present bacterial isolates and antimicrobial susceptibility which was expressed as percent of resistant, intermediate or susceptible.
The effect of different cultural conditions (incubation time, pH, temperature, nitrogen source, inoculum concentration, and carbon source) on the growth of the selected bacterial isolates, and the ability of the strain to produce biosurfactant was determined.
Overnight broth culture of the bacterial isolates and fungal isolates were seeded over the nutrient agar and potato dextrose agar plates respectively using sterile cotton swab to make lawn culture.
The authors had for aim to determine the distribution of bacterial isolates and the antibiotic susceptibility patterns of Escherichia coli from community-acquired urinary tract infections in an area covering 57,000 people (Elbeuf, Normandie, France).
