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The culturable bacterial fraction of 144 field samples (up to 864 analysis, including ampicillin enrichments) was analysed by PCR.
The bacterial fraction of soil was separated from the inorganic or humic layer by a differential centrifugation technique (14).
Metagenomics involves extracting the DNA from the bacterial fraction of faeces, applying whole-genome shotgun sequencing to build a reference gene catalogue and recording gene counts.
A recent study showed that the bacterial fraction of the gastrointestinal microbiota of the postinfectious irritable bowel syndrome patients have a distinct composition relative to controls, which most likely reflects a consequence of an intensive interaction between the ecosystem and the infectious agent (Jalanka-Tuovinen et al., 2013).
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No statistically significant differences were obtained in the stool culturable bacterial fractions of Lactobacillus, Bacteroides, or in the total anaerobe counts (data not shown).
Bacterial fractions of pUC19 transformed E. coli BL21 (DE3) served as negative controls.
The protozoal fraction contained a higher proportion of TVA and c9,t11-CLA than did the bacterial fraction, and dietary addition of SO, RO and LO resulted in a higher incorporation of TVA into both bacterial and protozoal microbial fractions, which probably positively affected TVA flow from the rumen.
These figures served for the choice of the optimal parameters for the assembly of the bacterial fraction.
Bacterial density was quantified following a 10 min period of static refrigeration to cause live nematodes, corpses, and fragments to sediment, and then by OD600 of an aliquot of the planktonic bacterial fraction in a cuvette.
For such avirulent strains, the optical density and colony forming units (cfu) of the bacterial fraction fell rapidly as nematodes grew.
The real-time PCR integrase assay was initially tested on a sample of concentrated bacterial fraction DNA from the same environment sampled for the metagenome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com