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Bacterial expression was performed essentially as described [ 45].
Induction of bacterial expression was performed with 1 mM IPTG for 3 to 5 hours.
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Transformation into a bacterial vector and subsequent protein expression was performed by Dr. Cynthia Kinsland.
Transformation into a bacterial vector and subsequent protein expression was performed by Dr. Cynthia Kinsland and the Cornell University Life Sciences Core Laboratories Center Protein Production Facility.
mRNA expression was performed as described16.
Bioreactor glucagon expression was performed.
Gene expression was performed using RT-PCR.
Protein expression was performed according to [26].
Briefly, bacterial expression was used to generate recombinant protein.
Evaluation of bacterial location was performed on three-dimensional z-stacks using ICY software.
Quantitative bacterial analysis of lung bacterial burden was performed according to a previously described technique [ 14].
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