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For bacterial examination 50 μl of milk or tenfold dilutions were incubated on blood agar plates for 24 hours at 37 °C and S. aureus colony counts were used to calculate the CFU per 50 μl milk.
However, results of the ordinary bacterial examination as well as anaerobes, fungi, and tuberculosis were negative.
Although we did not perform the bacterial examination on ascites on admission, S. pneumoniae was detected in the blood culture on the following day.
Although we hesitated to draw ascites for bacterial examination due to high risk of intraperitoneal bleeding at the puncture, it turned out that the patient had bacteremia by blood culture test performed on admission.
Priority must always be given to ensuring that routine bacterial examination is maintained whenever manpower and facilities are limited.
Patients with urine dipstick abnormalities as described above at baseline underwent a urine bacterial examination.
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These bacterial examinations in the water samples were carried out using the membrane filtration method as prescribed in the Standard Methods for the Examination of Water and Wastewater (APHA-AWWA-WEF 1998).
If lesions were observed by gross pathology, they were subjected to further bacterial examinations.
A final diagnosis, however, requires the demonstration of an anaerobe in bacterial culture examination.
No cause for the diarrhea was found on fecal bacterial culture; examination for ova, cysts, and parasites; and modified trichrome staining for microsporidia (Table 2).
Bacterial culture examination isolated a single species of anaerobe, Klebsiella pneumoniae, which was considered to be the cause of emphysematous cholecystitis, pneumobilia, and pneumoretroperitoneum.
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