Among different independently-evolved luminescent enzymatic apparati, the bacterial light emission system is best suitable for creation of autonomously luminescent plants due to cyanobacterial evolutionary origins of plant plastids [8], [9].
Bacterial light emission from LB plates or from tissue culture plates was captured using a ChemiDoc XRS system (Bio-Rad).
With the bacterial volatile emission profiles differing at the genus, species and strain levels [ 11, 42], it is likely that their volatile-mediated growth-promotion ability will vary.
Bacterial light-emission enzymatic system, encoded by the lux operon, is highly conserved among various species of luminous bacteria, with the most common architecture of the lux operon represented by luxCDABEG [2], [3].
However, according to the review of Schulz and Dickschat (2007) on bacterial VOC emissions, most of the substances we identified are known indicators for bacterial growth.
These include the strong near infrared absorbance by SWCNTs for tumour cell ablation [4], bacterial electroporation by field emission properties of MWCNTs [5] and localized heat release from SWCNTs following application of a radiofrequency radiation also for thermal ablation of cancer [6].
Among these interaction mechanisms, the emission of bacterial volatile organic compounds (VOCs) has been shown to promote plant growth [ 6] and VOC-mediated plant growth modulation is now widely considered to be an important mechanism [ 8].
In some cases charcoal stimulates bacterial growth, causing carbon emissions from soils to rise.
The chelating of metal co-factors (mainly copper, Cu) of the enzymes involved in the nitrification and denitrification steps was the probable mechanism for the reduction of N2O emissions as bacterial amoA, nirK, nirS and norB gene abundances, as well as the extractable Cu content, decreased in this treatment.
These findings stress the importance of proximity to sources of direct emissions of bacterial mutagens and imply that if important mutagen-forming atmospheric reactions occur, they likely occur in the winter and spring seasons as well as the photochemically more active summer and early fall periods.
