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Bacterial determination at two different time points revealed a reduction in the ability of both EA7F9 and IMT5155Δyqi to adhere to fibroblast cells up to about forty percent of the total adhesion by IMT5155 (Figure 3A).
During follow-up, two patients (6.7%) who were positive for bacterial determination suffered from CIED infection.
In the present study, the total positive rate for bacterial determination in 78 asymptomatic patients with CIED replacement reached 38.5%.
Recent research had indicated that means of sonic degradation were conducive to bacterial determination after replacement, removal, and infection [ 38– 40].
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Undiluted and 10-fold-diluted PF samples (0.1 ml) were plated on SBA plates to perform bacterial determinations.
Ileal digesta and feces were used for VFA and bacterial profile determination, respectively.
Quantitative assays were realized by bacterial killing determination using viable bacteria count through pour plate culture method.
Lungs were harvested 16 hr after infection for bacterial load determination.
These include detection of bacterial sialidases, determination of amine levels, and measurement of proline aminopeptidase activity [20], [21], [22].
Recently, we reported the high-throughput bacterial susceptibility determination, using the surface plasmon band shifts of gold nanoparticles [12].
This model is a very easy and convenient tool for bacterial virulence determination.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com