To know the bacterial community profiles at the genus level, we plotted a clustered heatmap.
The bacterial community profile in all mesocosms was monitored by LH-PCR over a period of two months.
A DGGE analysis of these PCR products was evaluated to ask if the extraction platform influenced the bacterial community profile, as extraction method bias is well documented in the literature for difficult sample types, such as those containing gram-positive bacteria (Frostegard et al.1999; Stach et al.2001).2001
Four continuous culture fermenters were used in a 4 × 4 Latin square design to test the hypothesis that fresh forage would increase flow of vaccenic acid (VA) from the fermenters compared with the same forage in hay form and that this difference would be diminished by adding sucrose to the hay diet by changing the bacterial community profile.
The bacterial community profiles of the same fecal samples studied here have previously been reported [12].
Results indicate that a minimum of 6 replicate samples are required in order to draw inferences on species, spatial or health-related changes in community composition, as a set of clearly distinct bacterial community profiles exist in healthy corals.
Various studies using PCR-DGGE and cluster analyses of human [16], [17] and murine GTMs [18], [19] for example, have indicated the occurrence of host-specific predominant mucosa-associated and luminal bacterial community profiles.
We describe in detail the distinct bacterial community profiles associated with young and old CF patients with a particular focus on the differences between respective "early" and "late" colonizing organisms.
The bacterial community profile of each of the three lobes were dominated in all three samples by the genus Pseudomonas (Figure 4A, Tissue), which was very similar to that of the BAL sample from our severe COPD subject (CS 4) and a number of others (Figure 2).
During chemotherapy, the bacterial community profile was altered in all patients.
