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Exact(8)
Bacterial colonies were selected on the basis of colony morphology and pigmentation.
The PCR amplified, crossover DNA fragment carrying the in-frame ∼2 kbp hupΔSL2 fusion allele was verified by DNA sequencing and introduced into the EcoRI site of plasmid pSUP202 (Table 1) by standard molecular cloning; E. coli strain MH3000 (Table 1) was subject to electroporation with recombinant plasmids, and transformed bacterial colonies were selected for tetracycline (Tc) resistance.
In order to isolate bacteria associated only with PWN, wood chips from infected wood samples from area Z were sterilized and processed as previously described and the bacterial colonies were selected only from the trails made by PWN on the medium.
Bacterial colonies were selected on the basis of color.
Transformed bacterial colonies were selected and used for large-scale expression of individual recombinant proteins.
The white bacterial colonies were selected, amplified and plasmids were extracted and purified using the QIAquick DNA reagent kit (Qiagen).
Similar(52)
Plates were then examined for bacterial growth and typical colonies were selected for identification.
Since there is no bacterial selection marker that differs for the bait and prey plasmids in this system, three to five colonies were selected and sequenced to identify the prey colonies.
Thirty-five white colonies were selected for each treatment and were subjected to colony PCR using primers 357f+GC-534r and ITS1F+GC-ITS2 for bacterial and fungal libraries, respectively.
Single colonies were selected for insert verification.
Positive colonies were selected by colony PCR.
More suggestions(18)
bacterial clones were selected
bacterial colonies were surveyed
bacterial infections were selected
bacterial colonies were pooled
bacterial progenies were selected
bacterial colonies were inoculated
bacterial chromosomes were selected
bacterial transformants were selected
bacterial colonies were screened
bacterial genomes were selected
bacterial isolates were selected
bacterial colonies were found
bacterial colonies were dissolved
bacterial colonies were scraped
bacterial colonies were seen
bacterial colonies were transferred
bacterial colonies were observed
bacterial colonies were subcultured
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