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Exact(20)
Bacterial cells were washed once in E3 medium containing 1/20 × LB.
Before sorting, bacterial cells were washed with phosphate-buffered saline (PBS) and diluted with PBS to an optical density of 0.03 at 600 nm.
The harvested bacterial cells were washed three times with saline solution (8.5 g/l KCl), and re-suspended in 50 mM potassium phosphate buffer (pH 7.5).
Bacterial cells were washed in PBS, resuspended in sample buffer (NuPage, Invitrogen) and disrupted using a Precellys 24 bead beater (three times 30 s, 6800 rpm; Bertin Technologies).
Bacterial cells were washed twice with PBS buffer, and pre-lysed with 250 µl of 50 mg/ml lysozyme and 120 µl of mutanolysin at 1000 units/ml (Sigma-Aldrich).
The subcultures were then grown for 3 h after which the bacterial cells were washed in PBS and used to infect HeLa and Intestine 407 cells at a multiplicity of infection of 1 1.
Similar(40)
At the end of 24 hr culture with bacterial sonicates, cells were washed, lysed and 40 µg of protein were run on a 10% SDS-PAGE and transferred onto a nitrocellulose membrane at 140 mA in 25 mM Tris-HCl, pH 8.3, 192 mM glycine, and 20% methanol.
To reduce overall bacterial numbers prior to DNA extractions, the cells were washed several times with water followed by low speed (300 g) centrifugation.
Lymphoid cells were washed.
Cells were washed with PBS.
The cells were washed twice.
More suggestions(17)
bacterial solutions were washed
bacterial cells were isolated
bacterial cells were extracted
bacterial cells were stained
bacterial cells were diluted
bacterial clumps were washed
bacterial cells were investigated
bacterial cells were resuspended
bacterial cells were distributed
bacterial colonies were washed
bacterial suspensions were washed
bacterial cells were inactivated
bacterial cells were observed
bacterial cells were homogenized
bacterial cultures were washed
bacterial cells were pelleted
bacterial cells were loaded
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