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Bacterial cells were fixed with 2.5% glutaraldehyde, post-fixed using repeated incubations with 1% osmium tetroxide/1% tannic acid, dehydrated with a graded ethanol series, critical-point dried and coated with 2 nm platinum.
Bacterial cells were fixed with 2.5 % glutaraldehyde in phosphate buffer saline (pH 7.2), incubated at room temperature for 30 min and washed thrice with Na-phosphate buffer.
G. vaginalis 14018 was grown to in culture media and bacterial cells were fixed on a glass chamber slide using 4% paraformaldehyde.
Bacterial cells were fixed with 8% glutaraldehyde for 1 hr.
For SEM analysis, Caco-2 cells cocultured with bacterial cells were fixed for 1 h with 2.5% glutaraldehyde in 0.1 M phosphate buffer (PB, pH 7.4) at room temperature.
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To detect endogenous Rab14 or bacterial IncG, infected cells were fixed in 3% PFA and permeabilized for 20 min with 0.2% saponin/BSA in PBS, then incubated for 1 h with the corresponding primary antibody followed by incubation with a fluorescent-labeled secondary antibody.
Additionally, cells were fixed to be viewed by FESEM to observe the bacterial morphological changes after treated with ZnO-NPs.
Bacterial docking was monitored by time lapse microscopy (phase contrast and GFP-fluorescence) and cells were fixed after 15 min of infection (Fig. 2B).
Seven days after infection, cells were fixed.
After incubation, the cells were fixed.
Cells were fixed at 4 DIV.
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