Total lipid contents of known weight of bacterial dry cells were extracted with mixture of chloroform/methanol (2 : 1 V/V) for 24 hr.
Total genomic DNA of K. pneumoniae and E. coli cells were extracted using the AxyPrep Bacterial Genomic DNA Miniprep kit.
RNA from bacteria and L929 cells were extracted simultaneously to avoid changes in transcriptomic profile after the bacterial purification process.
Instead, they urge that stem cells be extracted from adults.
Bacterial cells were lysed and genomic DNA was extracted, following the manufacturers protocol provided in the innuPREP Bacteria DNA Kit (Analytik Jena, Jena, Germany).
Bacterial cells were harvested directly from plates and plasmid DNA was extracted over four columns of PerfectPrep Endofree Maxi Kit (5 Prime, Hilden, Germany).
Cultured cells were harvested by centrifugation at 10,000×g, and the bacterial cell pellets were extracted with 3 ml of acetone containing 0.1% butylated hidroxy toluene (w/v), by the aid of sonication.
Agarose plugs were prepared from the cultures, and within the plugs, the bacterial cells were lysed by proteinase K. Extracted genomic DNA was digested with the restriction endonuclease SmaI [ 15, 16].
For isolation of periplasmic extracts, the bacterial cells were harvested by centrifugation at 4°C and resuspended in 1/10 initial volume of cold spheroplast buffer (20% sucrose, 50 mM Tris-HCl, 1 mM EDTA, pH 8.0).
The bacterial cells were grown in MSM media supplemented with 0.1% glucose, 0.4% yeast extract and 50 mg L−1 dye to enhance the production of azo-reductase enzyme.
