Sentence examples for bacterial cells were diluted from inspiring English sources

At the exponential growth phase, bacterial cells were diluted with sterile deionised water to the required cell density (104 CFU ml-1), as described by Rincon and Pulgarin (2003) and Gogniat et al. (2006).

The harvested bacterial cells were diluted in PBS to a final concentration of 5 × 107 cells per ml by measuring the OD of the bacterial suspension at 540 nm (an OD value of ~ 1 indicates 109 bacterial cells per ml).

Bacterial cells were diluted and Caco-2 cells were inoculated at an MOI of 10.

Overnight cultures of bacterial cells were diluted 50-fold into 1 L LB broth, supplemented with appropriate antibiotics, cultured as above until an OD600 of 0.4 0.6 was reached, and then induced to express the recombinant proteins for 4 h by adding isopropyl β -D-galactopyranoside (IPTG) to a final concentration of 0.6 mM.

When used for bacterial invasion studies, the cells were diluted 1 2, seeded in 24 well plates (Corning) and incubated over night at 37°C.

Yeast cells were diluted from 10 to 2.5 of OD600.

Cells were diluted 1 10 again before loading.

For inoculation, the fresh bacterial cells were resuspended and diluted in DMEM to a concentration about 6×107 CFU per ml.

Bacterial cells were resuspended in PBS, diluted to 5 9×103 CFU/ml and incubated in serum-free RPMI (Sigma) supplemented with 20% human serum and 1% DMSO or 100 µM C7.

To determine quantitatively the number of attached bacterial cells, lysates were diluted in TSB (tryptic soy broth) and plated onto TSA plates.

After incubation, bacterial cells were harvested by centrifugation and diluted to 5×10 CFU/ml (inf1, Pto, and AvrPto) and 2×10 CFU/ml (Cf9 and AvrCf9) for infiltration.